The Company has a strong expertise through the entire manufacturing process.

Based on theorical amino-acid sequences provided by its customers, BioProtein Technologies generates transgenic rabbit lines. These animals are ideal bioreactors for producing gram to dozens kilogram quantities of complex glycosylated proteins and vaccines within a framework that is fast, safe, and less expensive than other bioreactors.--> More…

BioProtein Technologies owns the relevant intellectual property and has developed the know-how that allows its researchers to quickly generate transgenic rabbits expressing defined proteins in their milk. In particular, four patent families protect the entire production process. These include the exclusive worldwide rights for the use of the rabbit WAP (Whey Acidic Protein) gene promoter, a key promoter for secreting proteins or vaccines in milk. --> More...

:::::::: Your Integrated Manufacturing Solution

From your selected amino acid sequence, BioProtein Technologies designs an optimized cDNA and generates transgenic rabbit lines expressing a biopharmaceutical of interest in their milk.

From this milk, the protein of interest can be extracted and purified to deliver up to dozens kilogram quantities of highly purified proteins both time- and cost-effectively.

Typically, 20 founder rabbits are generated in the standard process. Indeed, the low cost of rabbit embryos and the ability to transfer up to 20 embryos per recipient females allow the Company to generate a large number of transgenic founders for each project. This gives the opportunity to increase the chances to obtain several transgenic rabbit lines expressing sufficient quantities of biologically active proteins.

Founders are selected for their efficiency to produce the protein of interest in their milk and used to generate a second generation (F1) of transgenic rabbits. Transgenic F1 progeny is identified via tissue biopsy followed by a PCR analysis. Sexually mature transgenic F1 females are then inseminated with non-transgenic sperm. Their milk is mechanically collected and the recombinant protein is characterized in order to select the best offspring lines for large scale production and to develop the purification process strategy. In parallel, sperm or embryos banks are made in order to prepare Master and Working Transgenic Banks as required by the regulatory authorities.
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As a rabbit can produce between 10-15 litres of milk each year with an average of 8 young rabbits, the production scale-up can be quickly reached from an original group of founders.

The industrial purification under GMP conditions, is performed by our customers themselves, for those who have DSP capabilities in house. Alternatively, protein characterization and purification can be outsourced to CMOs in compliance with GMP standards.